Chinese Institute for Brain Research, Beijing CHENJIAN Lab

科学研究

1. Chenhui, Zhao., Xueyan, Hu., Xiudong, Guan., Xiaojun, Fu., Tingting, Wang., Mengyuan, Li., Xinze, Liu., Jiarui, Zhao., Di, Wu., Fan, Zhang., Jiaying, Fu., Jiang, Li., Tieqiang, Zhang., Xiaochun, Jiang., Changxiang, Yan., Wang, Jia., Ence, Yang., Jian, Chen.(2025). Molecular landscape, subtypes, and therapeutic vulnerabilities of central nervous system solitary fibrous tumors. Nat Commun, 16(1), 7870. doi:10.1038/s41467-025-63039-4

2. Di, Wu., Ying, Cao., Hongjie, Chen., Minghao, Du., Tingting, Wang., Jiarui, Zhao., Mengyuan, Li., Wenjing, Wu., Huixuan, Zhang., Ence, Yang., Jing, Yang., Jian, Chen.(2025). Common signatures of neutrophils in diverse disease conditions. Cell Discov, 11(1), 66. doi:10.1038/s41421-025-00818-9

3. Wang, T., Chen, J. Optimized Workflow for Isolation and Long-term Culture of Patient-derived Glioma Cells Retaining Original Tumor Characteristics. J. Vis. Exp. (221), e68566, doi: (2025).

4. Jingwei, Zhang., Yueqi, Jin., Haodong, Lin., Jiaming, Deng., Yan, Ju., Xueyan, Hu., Jianqi, She., Zhijian, Liang., Kongxu, Dai., Mantang, Qiu., Kunkun, Sun., Jun, Wang., Fan, Yang., Jian, Chen., Ence, Yang., Xiao, Li.(2025). Amplification of extrachromosomal MYC paralogs shapes immunosuppressive tumor microenvironment in small cell lung cancer. Clin Cancer Res, undefined(0), . doi:10.1158/1078-0432.CCR-24-3399

5. Fangfang, Ren., Yulan, Yi., Ting, Lu., Xinze, Liu., Gang, Cui., Song, Huang., Luis F, Parada., Jian, Chen.(2025). Synthetic lethality through Gsk3β inhibition in glioma stem cells via the WNT-WWC1-YAP axis. Oncogene, 0(0), 0. doi:10.1038/s41388-025-03418-9

6. Xing, Li., Wansong, Zhang., Yitong, Fang., Tianhu, Sun., Jian, Chen., Ruilin, Tian.(2025). Large-scale CRISPRi screens link metabolic stress to glioblastoma chemoresistance. J Transl Med, 23(1), 289. doi:10.1186/s12967-025-06261-4

7. Hongjie, Chen., Xin, Zhou., Tingting, Liu., Jiaqi, Liu., Di, Wu., Xia, Xu., Shanwu, Ma., Guangliang, Qiang., Jian, Chen., Ying, Cao., Wei, Fu., Jing, Yang.(2025). Postprandial parasympathetic signals promote lung type 2 immunity. Neuron, 113(5), 670-683.e7. doi:10.1016/j.neuron.2024.12.020

8. Zhao Jiarui., Wu Di., Liu Jiaqi., Zhang Yang., Li Chunzhao., Zhao Weichen., Cao Penghui., Wu Shixuan., Li Mengyuan., Li Wenlong., Liu Ying., Huang Yingying., Cao Ying., Sun Yiwen., Yang Ence., Ji Nan., Yang Jing., Chen Jian.(2024). Disease-specific suppressive granulocytes participate in glioma progression. Cell Rep, 43(12), 115014. doi:10.1016/j.celrep.2024.115014

9. Min, Wu*., Tingting, Wang*., Nan, Ji*., Ting, Lu*., Ran, Yuan*., Lingxiang, Wu*., Junxia, Zhang., Mengyuan, Li., Penghui, Cao., Jiarui, Zhao., Guanzhang, Li., Jianyu, Li., Yu, Li., Yujie, Tang., Zhengliang, Gao., Xiuxing, Wang., Wen, Cheng., Ming, Ge., Gang, Cui., Rui, Li., Anhua, Wu., Yongping, You#., Wei, Zhang#., Qianghu, Wang#., Jian, Chen#.(2024). Multi-omics and pharmacological characterization of patient-derived glioma cell lines. Nat Commun, 15(1), 0. doi:10.1038/s41467-024-51214-y

10. Jiaqi Liu, Mantang Qiu, Wenxiang Wang, Ying Cao, Fan Yang, Kezhong Chen, Jian Chen, Jing Yang.(2024). Sympathetic Signals Promote Immunosuppressive Neutrophils in Lung Tumors. bioRxiv . doi: https:// doi.org/10.1101/ 2024.02.15. 580434

11. Qun, Xu., Ying, Cao., Fanni, Kong., Jiaqi, Liu., Xin, Chen., Yifei, Zhao., Chin-Hui, Lai., Xin, Zhou., Hao, Hu., Wei, Fu., Jian, Chen., Jing, Yang.(2024). Multiple cancer cell types release LIF and Gal3 to hijack neural signals. Cell Res, 34(5), 345-354. doi:10.1038/s41422-024-00946-z

12. Ran, Yuan., Wei, Zhang., Yong-Ping, You., Gang, Cui., Zhengliang, Gao., Xiuxing, Wang., Jian, Chen.(2023). Vitamin D3 suppresses the cholesterol homeostasis pathway in patient-derived glioma cell lines. FEBS Open Bio, 13(9), 0. doi:10.1002/2211-5463.13679

13. Le, Wen., Xian-Zhang, Wang., Yong, Qiu., Yue-Peng, Zhou., Qing-Yang, Zhang., Shuang, Cheng., Jin-Yan, Sun., Xing-Jun, Jiang., Simon, Rayner., William J, Britt., Jian, Chen., Fei, Hu., Fang-Cheng, Li., Min-Hua, Luo., Han, Cheng. (2023). SOX2 downregulation of PML increases HCMV gene expression and growth of glioma cells. PLoS Pathog, 19(4), e1011316. doi:10.1371/journal.ppat.1011316

14. Chenchao, Yan., Yajing, Meng., Jie, Yang., Jian, Chen., Wei, Jiang.(2023). Translational landscape in human early neural fate determination. Development, 150(6), . doi:10.1242/dev.201177

15. Min, Wu., Lingxiang, Wu., Wei, Wu., Mengyan, Zhu., Jianyu, Li., Ziyu, Wang., Jie, Li., Rong, Ding., Yuan, Liang., Liangyu, Li., Tingting, Zhang., Bin, Huang., Yun, Cai., Kening, Li., Lu, Li., Rui, Zhang., Baoli, Hu., Fan, Lin., Xiuxing, Wang., Siyuan, Zheng., Jian, Chen., Yongping, You., Tao, Jiang., Junxia, Zhang., Hongshan, Chen., Qianghu, Wang.(2023). Phagocytosis of Glioma Cells Enhances the Immunosuppressive Phenotype of Bone Marrow-Derived Macrophages. Cancer Res, 83(5), 771-785. doi:10.1158/0008-5472.CAN-22-1570

16. Xia, Meng., Jian, Chen., Wenwen, Zeng.(2022). Stromal cell-derived NGF controls sympathetic innervation in subcutaneous fat. J Lipid Res, 64(2), 100264. doi:10.1016/j.jlr.2022.100264

17. Jianqi, She., Minghao, Du., Zhanzhan, Xu., Yueqi, Jin., Yu, Li., Daoning, Zhang., Changyu, Tao., Jian, Chen., Jiadong, Wang., Ence, Yang.(2022). The landscape of hervRNAs transcribed from human endogenous retroviruses across human body sites. Genome Biol, 23(1), 231. doi:10.1186/s13059-022-02804-w

18. Xianchun, Lan., Song, Ding., Tianzhe, Zhang., Ying, Yi., Conghui, Li., Wenwen, Jin., Jian, Chen., Kaiwei, Liang., Hengbin, Wang., Wei, Jiang.(2022). PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression. Nat Commun, 13(1), 4601. doi:10.1038/s41467-022-32295-z

19. Di, Wu., Qingshan, Chen., Jian, Chen.(2022). Case Report: Malignant Brain Tumors in Siblings With MSH6 Mutations. Front Oncol, 12(0), 920305. doi:10.3389/fonc.2022.920305

20. Jingjing, Wei., Di, Wu., Yu, Shao., Beibei, Guo., Jingjing, Jiang., Jian, Chen., Jinping, Zhang., Fenghua, Meng., Zhiyuan, Zhong.(2022). ApoE-mediated systemic nanodelivery of granzyme B and CpG for enhanced glioma immunotherapy. J Control Release, 347(0), 68-77. doi:10.1016/j.jconrel.2022.04.048

21. Jingjing, Wei., Di, Wu., Songsong, Zhao., Yu, Shao., Yifeng, Xia., Dawei, Ni., Xinyun, Qiu., Jinping, Zhang., Jian, Chen., Fenghua, Meng., Zhiyuan, Zhong.(2022). Immunotherapy of Malignant Glioma by Noninvasive Administration of TLR9 Agonist CpG Nano-Immunoadjuvant. Adv Sci (Weinh), 9(13), e2103689. doi:10.1002/advs.202103689

22. Jinchun, Chang., Chenjia, Guo., Jianyu, Li., Zhangqian, Liang., Yankai, Wang., Anliang, Yu., Runze, Liu., Yuting, Guo., Jian, Chen., Song, Huang.(2022). EN1 Regulates Cell Growth and Proliferation in Human Glioma Cells via Hedgehog Signaling. Int J Mol Sci, 23(3), . doi:10.3390/ijms23031123

23. Song, Ding., Xianchun, Lan., Yajing, Meng., Chenchao, Yan., Mao, Li., Xiang, Li., Jian, Chen., Wei, Jiang.(2021). CHD8 safeguards early neuroectoderm differentiation in human ESCs and protects from apoptosis during neurogenesis. Cell Death Dis, 12(11), 981. doi:10.1038/s41419-021-04292-5

24. Zelin, Liu., Changyu, Tao., Shiwei, Li., Minghao, Du., Yongtai, Bai., Xueyan, Hu., Yu, Li., Jian, Chen., Ence, Yang.(2021). circFL-seq reveals full-length circular RNAs with rolling circular reverse transcription and nanopore sequencing. Elife, 10(0), . doi:10.7554/eLife.69457

25. Bo, Wang., Peipei, Liu., Hui, Huang., Xiting, Wang., Mengling, Zhang., Jian, Huang., Fang, Lu., Jian, Chen., Yang, Liu., Zhenhui, Kang.(2021). Carbon dots up-regulate heme oxygenase-1 expression towards acute lung injury therapy. J Mater Chem B, 9(43), 9005-9011. doi:10.1039/d1tb01283e

26. Liao D*, Zhong L*, Yin J*, Zeng C, Wang X, Huang X, Chen J, Zhang H, Zhang R, Guan XY, Shuai X, Sui J, Gao S, Deng W, Zeng YX, Shen JN#, Chen J#, Kang T# （2020）Chromosomal translocation-derived aberrant Rab22a drives metastasis of osteosarcoma. Nat Cell Biol. 10.1038/s41556-020-0522-z

27. Yu, Chao., Chao, Liang., Huiquan, Tao., Yaran, Du., Di, Wu., Ziliang, Dong., Qiutong, Jin., Guobin, Chen., Jun, Xu., Zhisheng, Xiao., Qian, Chen., Chao, Wang., Jian, Chen., Zhuang, Liu.(2020). Localized cocktail chemoimmunotherapy after in situ gelation to trigger robust systemic antitumor immune responses. Sci Adv, 6(10), eaaz4204. doi:10.1126/sciadv.aaz4204

28. Zelin, Liu., Yuan, Ran., Changyu, Tao., Sichen, Li., Jian, Chen., Ence, Yang.(2019). Detection of circular RNA expression and related quantitative trait loci in the human dorsolateral prefrontal cortex. Genome Biol, 20(1), 99. doi:10.1186/s13059-019-1701-8

29. Yaran, Du., Ting, Lu., Song, Huang., Fangfang, Ren., Gang, Cui., Jian, Chen.(2018). Somatic mutation landscape of a meningioma and its pulmonary metastasis. Cancer Commun (Lond), 38(1), 16. doi:10.1186/s40880-018-0291-2

30. Rui, Shen., Peipei, Liu., Yiqiu, Zhang., Zhao, Yu., Xuyue, Chen., Lu, Zhou., Baoqing, Nie., Anna, Żaczek., Jian, Chen., Jian, Liu.(2018). Sensitive Detection of Single-Cell Secreted H(2)O(2) by Integrating a Microfluidic Droplet Sensor and Au Nanoclusters. Anal Chem, 90(7), 4478-4484. doi:10.1021/acs.analchem.7b04798

31. Lili, Qi., Lixin, Zhou., Mingmin, Lu., Kehu, Yuan., Zhongwu, Li., Guiyin, Wu., Xiaozheng, Huang., Yi, Shen., Min, Zhao., Wei, Fu., Boyang, Chu., Guangli, Wang., Fangfang, Ren., Donghui, Ma., Jian, Chen.(2017). Development of a highly specific HER2 monoclonal antibody for immunohistochemistry using protein microarray chips. Biochem Biophys Res Commun, 484(2), 248-254. doi:10.1016/j.bbrc.2017.01.086

32. Sheila R, Alcantara Llaguno., Zilai, Wang., Daochun, Sun., Jian, Chen., Jing, Xu., Euiseok, Kim., Kimmo J, Hatanpaa., Jack M, Raisanen., Dennis K, Burns., Jane E, Johnson., Luis F, Parada.(2015). Adult Lineage-Restricted CNS Progenitors Specify Distinct Glioblastoma Subtypes. Cancer Cell, 28(4), 429-440. doi:10.1016/j.ccell.2015.09.007

33. Sanchez-Ortiz E, Cho W, Nazarenko I, Mo W, Chen J#, Parada LF# (2014).NF1 regulation of RAS/ERK signaling is required for appropriate granule neuron progenitor expansion and migration in cerebellar development. Genes Dev. 28(21) : 2407-20 . (# Corresponding author)

34. Wei, Mo., Jian, Chen., Amish, Patel., Liang, Zhang., Vincent, Chau., Yanjiao, Li., Woosung, Cho., Kyun, Lim., Jing, Xu., Alexander J, Lazar., Chad J, Creighton., Svetlana, Bolshakov., Renée M, McKay., Dina, Lev., Lu Q, Le., Luis F, Parada.(2013). CXCR4/CXCL12 mediate autocrine cell- cycle progression in NF1-associated malignant peripheral nerve sheath tumors. Cell, 152(5), 1077-90. doi:10.1016/j.cell.2013.01.053

35. Chen J, Li Y, Yu TS, McKay RM, Burns DK, Kernie SG, Parada LF (2012). A restricted cell population propagates glioblastoma growth after chemotherapy. Nature. 488(7412):522-6.

36. Chen J, Mckay RM, Parada LF (2012). Malignant glioma: lessons from genomics, mouse models, and stem cells. Cell. 30;149(1):36-47.

37. Alcantara Llaguno S*, Chen J*, Kwon CH*, Jackson EL, Li Y, Burns DK, Alvarez-Buylla A, Parada LF (2009). Malignant astrocytomas originate from neural stem/progenitor cells in a somatic tumor suppressor mouse model. Cancer Cell. 15:45-56. (, Authorship equally shared)

38. Cory A, Blaiss., Tzong-Shiue, Yu., Gui, Zhang., Jian, Chen., Georgi, Dimchev., Luis F, Parada., Craig M, Powell., Steven G, Kernie.(2011). Temporally specified genetic ablation of neurogenesis impairs cognitive recovery after traumatic brain injury. J Neurosci, 31(13), 4906-16. doi:10.1523/JNEUROSCI.5265-10.2011

39. Sheila R, Alcantara Llaguno., Jian, Chen., Luis F, Parada.(2009). Signaling in malignant astrocytomas: role of neural stem cells and its therapeutic implications. Clin Cancer Res, 15(23), 7124-9. doi:10.1158/1078-0432.CCR-09-0433

40. Lu, Lin., Jian, Chen., James A, Richardson., Luis F, Parada.(2009). Mice lacking neurofibromin develop gastric hyperplasia. Am J Physiol Gastrointest Liver Physiol, 297(4), G751-61. doi:10.1152/ajpgi.00007.2009

41. Jian, Chen., Chang-Hyuk, Kwon., Lu, Lin., Yanjiao, Li., Luis F, Parada.(2009). Inducible site-specific recombination in neural stem/progenitor cells. Genesis, 47(2), 122-31. doi:10.1002/dvg.20465

42. Sheila, Alcantara Llaguno., Jian, Chen., Chang-Hyuk, Kwon., Erica L, Jackson., Yanjiao, Li., Dennis K, Burns., Arturo, Alvarez-Buylla., Luis F, Parada.(2008). Malignant astrocytomas originate from neural stem/progenitor cells in a somatic tumor suppressor mouse model. Cancer Cell, 15(1), 45-56. doi:10.1016/j.ccr.2008.12.006

43. Yun, Li., Bryan W, Luikart., Shari, Birnbaum., Jian, Chen., Chang-Hyuk, Kwon., Steven G, Kernie., Rhonda, Bassel-Duby., Luis F, Parada.(2008). TrkB regulates hippocampal neurogenesis and governs sensitivity to antidepressive treatment. Neuron, 59(3), 399-412. doi:10.1016/j.neuron.2008.06.023

44. Chang-Hyuk, Kwon., Dawen, Zhao., Jian, Chen., Sheila, Alcantara., Yanjiao, Li., Dennis K, Burns., Ralph P, Mason., Eva Y-H P, Lee., Hong, Wu., Luis F, Parada.(2008). Pten haploinsufficiency accelerates formation of high-grade astrocytomas. Cancer Res, 68(9), 3286-94. doi:10.1158/0008-5472.CAN-07-6867

45. Mark E, Lush., Yun, Li., Chang-Hyuk, Kwon., Jian, Chen., Luis F, Parada.(2008). Neurofibromin is required for barrel formation in the mouse somatosensory cortex. J Neurosci, 28(7), 1580-7. doi:10.1523/JNEUROSCI.5236-07.2008

46. S Alcantara, Llaguno., J, Chen., C-H, Kwon., L F, Parada.(2008). Neural and cancer stem cells in tumor suppressor mouse models of malignant astrocytoma. Cold Spring Harb Symp Quant Biol, 73(0), 421-6. doi:10.1101/sqb.2008.73.005

47. Ling, Yi., Zhengquan, Li., Kehu, Yuan., Xiuxia, Qu., Jian, Chen., Guangwen, Wang., Hong, Zhang., Hongpeng, Luo., Lili, Zhu., Pengfei, Jiang., Lirong, Chen., Yan, Shen., Min, Luo., Guoying, Zuo., Jianhe, Hu., Deliang, Duan., Yuchun, Nie., Xuanling, Shi., Wei, Wang., Yang, Han., Taisheng, Li., Yuqing, Liu., Mingxiao, Ding., Hongkui, Deng., Xiaojie, Xu.(2004). Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells. J Virol, 78(20), 11334-9. doi:10.1128/JVI.78.20.11334-11339.2004

48. Yuchun, Nie., Peigang, Wang., Xuanling, Shi., Guangwen, Wang., Jian, Chen., Aihua, Zheng., Wei, Wang., Zai, Wang., Xiuxia, Qu., Min, Luo., Lei, Tan., Xijun, Song., Xiaolei, Yin., Jianguo, Chen., Mingxiao, Ding., Hongkui, Deng.(2004). Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression. Biochem Biophys Res Commun, 321(4), 994-1000. doi:10.1016/j.bbrc.2004.07.060

49. Kehu, Yuan., Ling, Yi., Jian, Chen., Xiuxia, Qu., Tingting, Qing., Xi, Rao., Pengfei, Jiang., Jianhe, Hu., Zikai, Xiong., Yuchun, Nie., Xuanling, Shi., Wei, Wang., Chen, Ling., Xiaolei, Yin., Keqiang, Fan., Luhua, Lai., Mingxiao, Ding., Hongkui, Deng.(2004). Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein. Biochem Biophys Res Commun, 319(3), 746-52. doi:10.1016/j.bbrc.2004.05.046

50. Peigang, Wang., Jian, Chen., Aihua, Zheng., Yuchun, Nie., Xuanling, Shi., Wei, Wang., Guangwen, Wang., Min, Luo., Huijun, Liu., Lei, Tan., Xijun, Song., Zai, Wang., Xiaolei, Yin., Xiuxia, Qu., Xiaojing, Wang., Tingting, Qing., Mingxiao, Ding., Hongkui, Deng.(2004). Expression cloning of functional receptor used by SARS coronavirus. Biochem Biophys Res Commun, 315(2), 439-44. doi:10.1016/j.bbrc.2004.01.076

51. Wang P*, Chen J*, Zheng A*, Nie Y, Shi X, Wang W, Wang G, Luo M, Liu H, Tan L, Song X, Wang Z, Yin X, Qu X, Wang X, Qing T, Ding M, Deng H (2004). Expression cloning of functional receptor used by SARS coronavirus. Biochem Biophys Res Commun. 315:439-44. (,Authorship equally shared)